培养的小鼠脊髓神经元中的毒蕈碱敏感性电压依赖性钾电流

Muscarine-sensitive voltage-dependent potassium current in cultured murine spinal cord neurons.

作者信息

Nowak L M, Macdonald R L

出版信息

Neurosci Lett. 1983 Jan 31;35(1):85-91. doi: 10.1016/0304-3940(83)90531-1.

Abstract

Muscarine produced membrane depolarization and decreased membrane conductance of mouse spinal cord neurons in dissociated cell culture. When the neurons were voltage clamped, muscarine evoked inward currents which increased with membrane depolarization and decreased with membrane hyperpolarization. However, the muscarine-induced inward currents did not invert at large negative potentials, suggesting that muscarine decreased a voltage-dependent potassium current (m-current) [2]. Using the voltage-jump current-relaxation technique, m-current was demonstrated in spinal cord neurons and shown to be a muscarine-sensitive potassium current.

摘要

毒蕈碱可使离体培养的小鼠脊髓神经元发生膜去极化并降低膜电导。当对这些神经元进行电压钳制时，毒蕈碱可诱发内向电流，该电流随膜去极化而增加，随膜超极化而减小。然而，毒蕈碱诱导的内向电流在大的负电位时并不反转，这表明毒蕈碱降低了一种电压依赖性钾电流（m电流）[2]。使用电压跃变电流松弛技术，在脊髓神经元中证实了m电流，并表明其是一种对毒蕈碱敏感的钾电流。