Absence of pH effect on creatine kinase leakage from mouse skeletal muscle.

Skeletal muscle enzyme leakage has been found to be independent of extracellular pH (6.6 - 8.2), changes in ionic strength, osmolarity, and the weight changes that occur during in vitro incubation. This is in contrast to the effect of pH and ionic strength on heart, and no change in heart weight, during incubation in similar media. These observations further emphasize the disparate responses of heart and skeletal muscle to changes in the extracellular environment.