Chun Y H, Johnson E M, Gabel B E
Teratology. 1983 Feb;27(1):95-100. doi: 10.1002/tera.1420270114.
Three milligrams of vinblastine sulfate per liter of culture medium prevented normal development of all reaggregated artificial hydra "embryos" at the 66-hour stage of regeneration. One milligram of vinblastine per liter of medium had no effect on development, and 100 mg did not markedly accelerate the developmental disruption beyond the pace of the 2 mg/liter concentration which was the lowest effective concentration. Treatment with vinblastine through the first few hours of development was sufficient to produce the toxic endpoint at 66 hours of incubation, but effects were delayed in appearance when treatment was initiated later in development. Early development of exposed preparations proceeded normally even in the presence of rather high concentrations of the test substance, and the pellet was resistant to the effects of vinblastine treatment begun after 24 hours of development. From these results, it would appear that interference with cell division is not the primary means by which vinblastine perturbs this developing system.
每升培养基中加入3毫克硫酸长春碱可在再生66小时阶段阻止所有重新聚集的人工水螅“胚胎”正常发育。每升培养基中加入1毫克长春碱对发育没有影响，而加入100毫克也不会比2毫克/升浓度（最低有效浓度）更显著地加速发育破坏。在发育最初几个小时用长春碱处理足以在培养66小时时产生毒性终点，但如果在发育后期开始处理，效果会延迟出现。即使存在相当高浓度的受试物质，暴露制剂的早期发育仍正常进行，并且在发育24小时后开始的长春碱处理对细胞团没有影响。从这些结果来看，干扰细胞分裂似乎不是长春碱扰乱这个发育系统的主要方式。
