Birt D F, Hruza D S, Baker P Y
Toxicol Appl Pharmacol. 1983 Mar 30;68(1):77-86. doi: 10.1016/0041-008x(83)90356-3.
Syrian hamsters were fed one of three levels of lactalbumin (10, 20, or 40%) from 4 weeks of age. Parents (F0 generation) and their offspring (F1 generation) remained for life on the parental diet. Randomly selected hamsters from both generations were killed at 0, 6, 18, 30, and 60 weeks to evaluate hepatic microsomal drug metabolism systems. Microsomal protein content rose with increased dietary protein in both generations of females at 30 and 60 weeks and in both generations of males at 18 weeks. Microsomal protein rose during life, but the timing and magnitude of the increase varied with sex, diet, and generation. Cytochrome P-450 content increased with elevation in dietary protein at most measurement times in females and at all measurement times in F1 generation males. Hepatic aryl hydrocarbon hydroxylase (AHH) and aniline hydroxylase (ANH) activities were influenced by dietary protein, primarily at 6, 18, and 30 weeks, and these values tended to decline between maturity and senescence. AHH generally increased as dietary protein rose, except at 30 weeks, at which time the group fed the medium protein level usually had the highest activity. ANH activity was either elevated in groups fed the 20 or 40% lactalbumin levels or depressed in these groups at 6 and 18 weeks when these values were influenced by dietary protein. The effects depended upon sex and generation. Age effects on hepatic microsomal metabolism in hamsters varied by sex and dietary protein and were somewhat different than those reported for other rodents.
从4周龄起，给叙利亚仓鼠喂食三种水平（10%、20%或40%）的乳白蛋白中的一种。亲代（F0代）及其后代（F1代）终生维持亲代饮食。在0、6、18、30和60周时，随机选取两代中的仓鼠处死，以评估肝脏微粒体药物代谢系统。在30周和60周时，两代雌性仓鼠以及在18周时，两代雄性仓鼠的微粒体蛋白含量均随饮食蛋白增加而升高。微粒体蛋白在整个生命过程中都有所增加，但增加的时间和幅度因性别、饮食和代次而异。在大多数测量时间点，雌性仓鼠以及F1代雄性仓鼠在所有测量时间点的细胞色素P - 450含量均随饮食蛋白升高而增加。肝脏芳烃羟化酶（AHH）和苯胺羟化酶（ANH）活性受饮食蛋白影响，主要在6、18和30周时，这些值在成熟和衰老之间趋于下降。除了在30周时，AHH通常随饮食蛋白增加而升高，此时喂食中等蛋白水平的组通常具有最高活性。在6周和18周时，当这些值受饮食蛋白影响时，喂食20%或40%乳白蛋白水平的组中，ANH活性要么升高，要么降低。这些影响取决于性别和代次。年龄对仓鼠肝脏微粒体代谢的影响因性别和饮食蛋白而异，并且与其他啮齿动物的报道有些不同。
