马来亚眼镜蛇（Naja naja sputatrix）抗蛇毒血清的改进。

Improvement of Malayan cobra (Naja naja sputatrix) antivenin.

作者信息

Tan N H

出版信息

Toxicon. 1983;21(1):75-9. doi: 10.1016/0041-0101(83)90051-x.

Abstract

A low molecular weight toxic fraction was isolated from venom of the Malayan cobra (Naja naja sputatrix) by Sephadex G-50 gel filtration chromatography. The fraction accounted for almost 100% of the venom lethality. Antisera were prepared by immunizing rabbits with the low molecular weight toxic fraction, the glutaraldehyde-treated low molecular weight toxic fraction and the glutaraldehyde-treated, sea snake neurotoxin-enriched low molecular weight toxic fraction, respectively. Only the serum of rabbits immunized with the glutaraldehyde-treated, neurotoxin-enriched fraction gave effective protection against high doses of Malayan cobra venom. This antiserum is thus a potent Malayan cobra antivenin. One milliliter of this antiserum was able to neutralize 1.5 mg of Malayan cobra venom. It is thus 4-8 times more potent than the commercially available Malayan cobra antivenins produced by immunizing horses with whole Malayan cobra venom.

摘要

通过葡聚糖凝胶G - 50凝胶过滤色谱法从马来亚眼镜蛇（Naja naja sputatrix）的毒液中分离出一种低分子量毒性组分。该组分几乎占毒液致死性的100%。分别用低分子量毒性组分、经戊二醛处理的低分子量毒性组分以及经戊二醛处理且富含海蛇神经毒素的低分子量毒性组分免疫兔子，制备抗血清。只有用经戊二醛处理且富含神经毒素的组分免疫的兔子血清对高剂量的马来亚眼镜蛇毒液有有效的保护作用。因此，这种抗血清是一种有效的马来亚眼镜蛇抗蛇毒血清。1毫升这种抗血清能够中和1.5毫克马来亚眼镜蛇毒液。因此，它的效力比用整个马来亚眼镜蛇毒液免疫马匹生产的市售马来亚眼镜蛇抗蛇毒血清强4 - 8倍。