Sukhomlinov B F, Fedorovich A N, Chaĭka Ia P
Ukr Biokhim Zh (1978). 1983 Mar-Apr;55(2):199-201.
A procedure is suggested for obtaining intact enterocytes from rat duodenum mucosa by the tissue trypsinization in the isotonic Krebs solution (pH 7.9). The maximum yield of certain enterocytes is reached at the enzyme concentration of 0.03%; the incubation time--15 min; 200-250 mg of mucosa produces 3,7 X 10(6)--4,7 X 10(6) cells.
建议采用一种方法,即在等渗的克雷布斯溶液(pH 7.9)中通过组织胰蛋白酶消化从大鼠十二指肠黏膜获取完整的肠上皮细胞。在酶浓度为0.03%时可获得特定肠上皮细胞的最大产量;孵育时间为15分钟;200 - 250毫克黏膜可产生3.7×10⁶ - 4.7×10⁶个细胞。