Long term cultures of neural retina and pigment epithelium from newborn rabbits.

In vitro cultures of neural retina, obtained after dispersion and trypsinization of tissue fragments, were composed of 3 morphologically distinct types of neural cells, as demonstrated by silver impregnation. They resembled ganglion or receptor cells, horizontal or amacrine cells, and bipolar cells of the intact retina. Pigment epithelium was cultured without trypsinization. Both kinds of techniques may prove helpful for long term experiments in neurobiology and neurovirology.