Tsukamoto T, Ludwig H
Z Naturforsch C Biosci. 1983 Jan-Feb;38(1-2):141-5. doi: 10.1515/znc-1983-1-224.
In vitro cultures of neural retina, obtained after dispersion and trypsinization of tissue fragments, were composed of 3 morphologically distinct types of neural cells, as demonstrated by silver impregnation. They resembled ganglion or receptor cells, horizontal or amacrine cells, and bipolar cells of the intact retina. Pigment epithelium was cultured without trypsinization. Both kinds of techniques may prove helpful for long term experiments in neurobiology and neurovirology.
经组织碎片分散和胰蛋白酶消化后获得的神经视网膜体外培养物，由3种形态上不同类型的神经细胞组成，银浸染法证实了这一点。它们类似于完整视网膜的神经节或感受器细胞、水平或无长突细胞以及双极细胞。色素上皮未经胰蛋白酶消化进行培养。这两种技术可能对神经生物学和神经病毒学的长期实验都有帮助。
