Wierzchowski J, Shugar D
Z Naturforsch C Biosci. 1983 Jan-Feb;38(1-2):67-73. doi: 10.1515/znc-1983-1-213.
The nucleoside antibiotic formycin, 7-amino-3-(beta-D-ribofuranosyl)pyrazolo(4,3-d)pyrimidine, a structural analogue of adenosine, is deaminated about 10-fold faster by adenosine deaminase than adenosine itself, and is therefore a superior substrate for both routine assays and kinetic studies with the purified enzyme. The luminescence properties of formycin have been profited from to develop a fluorimetric assay for adenosine deaminase which is considerably more sensitive than the spectrophotometric procedure widely employed with adenosine as substrate. Examples are presented of its application to routine assays of adenosine deaminase levels in cellular extracts, as well as to kinetic studies with the purified enzyme, including the properties of some pyrazolopyrimidine and purine substrates and inhibitors.
核苷类抗生素助间型霉素，即7-氨基-3-(β-D-呋喃核糖基)吡唑并[4,3-d]嘧啶，是腺苷的结构类似物，被腺苷脱氨酶脱氨的速度比腺苷本身快约10倍，因此对于使用纯化酶进行的常规测定和动力学研究而言，它是一种更优质的底物。助间型霉素的发光特性已被用于开发一种腺苷脱氨酶的荧光测定法，该方法比广泛使用腺苷作为底物的分光光度法灵敏得多。文中给出了其在细胞提取物中腺苷脱氨酶水平常规测定以及纯化酶动力学研究中的应用实例，包括一些吡唑并嘧啶和嘌呤底物及抑制剂的特性。
