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The measurement of the kinetics of lipid phase transitions: a volume-perturbation kinetic calorimeter.

作者信息

Johnson M L, Winter T C, Biltonen R L

出版信息

Anal Biochem. 1983 Jan;128(1):1-6. doi: 10.1016/0003-2697(83)90335-4.

DOI:10.1016/0003-2697(83)90335-4
PMID:6846787
Abstract

A volume-perturbation kinetic calorimeter to be used to study membrane phase transitions in lipid vesicles has been developed. In this instrument, the voltage-dependent extension of a stack of piezoelectric crystals is used to force the solution being studied to undergo a small adiabatic bidirectional volume change. This volume change induces a shift in the equilibrium position of the lipid gel to liquid-crystalline transition. The time course of the relaxation to the new equilibrium position is then monitored by observing the induced temperature and pressure changes as a function of time.

摘要

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