Hintz C S, Chi M M, Lowry O H
Anal Biochem. 1983 Jan;128(1):186-90. doi: 10.1016/0003-2697(83)90361-5.
An enzymatic cycle for NADP which uses as one of its enzymes glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has occasionally caused trouble due to failure to completely heat-kill this enzyme before the indicator step. It was found that a very small increase in pH was the cause of this. It was also found that the other two proteins present in the reagent greatly increase heat inactivation of the enzyme. The inactivation problem is completely overcome by keeping the pH below 7.2.
一种用于NADP的酶循环，其使用来自嗜温明串珠菌的6-磷酸葡萄糖脱氢酶作为其中一种酶，由于在指示步骤之前未能完全热灭活该酶，偶尔会出现问题。发现pH值非常小的升高是造成这种情况的原因。还发现试剂中存在的其他两种蛋白质大大增加了该酶的热失活。通过将pH值保持在7.2以下，可完全克服失活问题。
