Burnett D, Chandy K G
Anal Biochem. 1983 Feb 1;128(2):317-22. doi: 10.1016/0003-2697(83)90381-0.
A simple method is described for the analysis of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with crossed immunoelectrophoresis. The technique allows the identification of specific proteins and estimation of their molecular weights after the electrophoresis of complex mixtures such as blood plasma. Human ceruloplasmin was estimated to have a molecular weight of 128,000 using this method. Reduction of plasma with 2-mercaptoethanol in the presence of protease inhibitors did not result in dissociation of ceruloplasmin into detectable smaller subunits, suggesting that native ceruloplasmin is composed of a single polypeptide chain.
本文描述了一种通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳结合交叉免疫电泳来分析蛋白质的简单方法。该技术可在对诸如血浆等复杂混合物进行电泳后，鉴定特定蛋白质并估计其分子量。使用此方法估计人铜蓝蛋白的分子量为128,000。在蛋白酶抑制剂存在的情况下，用2-巯基乙醇还原血浆并不会导致铜蓝蛋白解离成可检测到的较小亚基，这表明天然铜蓝蛋白由单条多肽链组成。
