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本文引用的文献

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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
Separation and purification of sarcosine dehydrogenase and dimethylglycine dehydrogenase.肌氨酸脱氢酶和二甲基甘氨酸脱氢酶的分离与纯化。
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3
On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.关于辅酶A的脂肪酰衍生物脱氢作用的机制。II. 电子传递黄素蛋白。
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Purification and properties of rat liver acyl-CoA dehydrogenases and electron transfer flavoprotein.大鼠肝脏酰基辅酶A脱氢酶和电子传递黄素蛋白的纯化及性质
J Biochem. 1981 Dec;90(6):1739-50. doi: 10.1093/oxfordjournals.jbchem.a133651.
5
Isovaleryl-CoA dehydrogenase: demonstration in rat liver mitochondria by ion exchange chromatography and isoelectric focusing.异戊酰辅酶A脱氢酶：通过离子交换色谱法和等电聚焦法在大鼠肝脏线粒体中的展示。
Proc Natl Acad Sci U S A. 1980 May;77(5):2646-50. doi: 10.1073/pnas.77.5.2646.
6
The effect of tetrahydrofolate on the reduction of electron transfer flavoprotein by sarcosine and dimethylglycine dehydrogenases.四氢叶酸对肌氨酸和二甲基甘氨酸脱氢酶还原电子传递黄素蛋白的影响。
Biochem J. 1982 Jun 1;203(3):707-15. doi: 10.1042/bj2030707.
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Spectroscopic determination of tryptophan and tyrosine in proteins.蛋白质中色氨酸和酪氨酸的光谱测定
Biochemistry. 1967 Jul;6(7):1948-54. doi: 10.1021/bi00859a010.
8
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
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Enzymatic properties of the inner and outer membranes of rat liver mitochondria.大鼠肝脏线粒体内膜和外膜的酶学特性
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猪肝线粒体中的电子传递黄素蛋白。一种简单的纯化方法及对某些分子特性的重新评估。

Electron transfer flavoprotein from pig liver mitochondria. A simple purification and re-evaluation of some of the molecular properties.

作者信息

Husain M, Steenkamp D J

出版信息

Biochem J. 1983 Feb 1;209(2):541-5. doi: 10.1042/bj2090541.

DOI:10.1042/bj2090541
PMID:6847633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154123/
Abstract

Electron transfer flavoprotein (ETF) from pig liver mitochondria has been purified to homogeneity by a three-step procedure with approx. 10-fold higher yields than previously reported. The purified ETF exhibits an absorption coefficient for the bound FAD of 13.5 mM-1.cm-1 at 436 nm and an isoelectric point of 6.75. Gel filtration, sodium dodecyl sulphate gel electrophoresis and flavin analysis indicate that pig liver ETF is a dimer, composed of non-identical subunits (Mr 38 000 and 32 000) with only one FAD/dimer. Anaerobic reduction by dithionite produces anionic flavin semiquinone as a stable intermediate and establishes the flavin to be the only redox-active chromophore in ETF.

摘要

通过三步纯化程序，猪肝线粒体中的电子传递黄素蛋白（ETF）已被纯化至均一状态，产率比之前报道的高约10倍。纯化后的ETF在436nm处结合FAD的吸收系数为13.5 mM-1.cm-1，等电点为6.75。凝胶过滤、十二烷基硫酸钠凝胶电泳和黄素分析表明，猪肝ETF是一种二聚体，由不相同的亚基（Mr 38 000和32 000）组成，每个二聚体仅含一个FAD。连二亚硫酸盐的厌氧还原产生阴离子黄素半醌作为稳定中间体，并确定黄素是ETF中唯一具有氧化还原活性的发色团。