影响可溶性多巴胺敏感腺苷酸环化酶的因素。

Factors affecting solubilized dopamine-sensitive adenylate cyclase.

作者信息

Marshall A M, Ramwani J, Mishra R K

出版信息

Biochem Pharmacol. 1983 Apr 1;32(7):1237-41. doi: 10.1016/0006-2952(83)90277-0.

Abstract

Bovine striatal adenylate cyclase was solubilized with sodium cholate and assayed for its responsiveness to a variety of agents. Magnesium ions (0-20 mM), guanylyl-imidodiphosphate (10-50 microM), and striatal lipids were effective in increasing enzyme activity. The adenylate cyclase could be stimulated by dopamine, and neuroleptic drugs inhibited the effect of dopamine (50 microM) with potencies that paralleled their clinical potencies. The IC50 values for spiroperidol, haloperidol and chlorpromazine were 0.2, 3 and 500 nM respectively.

摘要

牛纹状体腺苷酸环化酶用胆酸钠增溶，并检测其对多种试剂的反应性。镁离子（0 - 20 mM）、鸟苷酰亚胺二磷酸（10 - 50 μM）和纹状体脂质可有效提高酶活性。腺苷酸环化酶可被多巴胺刺激，抗精神病药物抑制多巴胺（50 μM）的作用，其效力与临床效力平行。螺哌啶醇、氟哌啶醇和氯丙嗪的IC50值分别为0.2、3和500 nM。