Ibrahiem E H, Nigam V N, Brailovsky C A, Madarnas P, Elhilali M
Cancer Res. 1983 Feb;43(2):617-22.
Primary bladder tumors induced in Fischer 344 inbred rats by N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide were transplanted in syngeneic rats by the intravesical, s.c., i.v., and orthotopic routes. Attempts were made to establish bladder cancer cell lines in vitro. No success was achieved in transplantation by either the s.c., i.v., or intravesical routes when primary tumor cells were transplanted as cell suspensions. Cell suspensions of primary tumors also failed to grow in culture. However, orthotopic implantation into the bladder submucosa gave 45% success. Tumor fragments obtained from either the primary tumor or its lung metastases resulted in 10.6 and 36% tumor takes, respectively, when implanted s.c. However, after one orthotopic passage in the bladder submucosa, the tumor cells injected as cell suspension grew s.c. in 14% and orthotopically in 79% of the animals. Tumor fragments obtained from orthotopic tumors and implanted s.c. resulted in 15% tumor takes. After the second orthotopic passage, tumor cells could be grown in cultures and orthotopically in 100% of animals. The technique of orthotopic implantation as well as the usefulness of this tumor model for bladder cancer studies are described.
用N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺在Fischer 344近交系大鼠中诱导产生的原发性膀胱肿瘤，通过膀胱内、皮下、静脉内和原位途径移植到同基因大鼠体内。尝试在体外建立膀胱癌细胞系。当将原发性肿瘤细胞作为细胞悬液进行移植时，通过皮下、静脉内或膀胱内途径均未成功。原发性肿瘤的细胞悬液在培养中也未能生长。然而，将肿瘤原位植入膀胱黏膜下层的成功率为45%。当将从原发性肿瘤或其肺转移灶获得的肿瘤组织块皮下植入时，肿瘤形成率分别为10.6%和36%。然而，在膀胱黏膜下层进行一次原位传代后，作为细胞悬液注射的肿瘤细胞在14%的动物皮下生长，在79%的动物原位生长。从原位肿瘤获得并皮下植入的肿瘤组织块，肿瘤形成率为15%。在第二次原位传代后，肿瘤细胞能够在培养中生长，并且在100%的动物原位生长。本文描述了原位植入技术以及该肿瘤模型在膀胱癌研究中的应用价值。
