Vinogradova N A, Nikol'skiĭ N N, Troshin A S
Tsitologiia. 1978 Mar;20(3):315-20.
In the experiments on isolated frog sartorius muscles, amines and amides were found to inhibit the process of stimulation of D-xylose transport induced by insulin, 2,4-dinitrophenol or potassium contracture. The inhibitory action was produced by urea, acetamide, guanidine, NH4Cl, mono-, di- and trimethyl- or ethylamines, some diamines (all the substances being, applied in the concentration range equal to 100 mM). The similar effect was obtained when cystamine (20 mM), tryptamine, 5-methoxytryptamine (2 mM) and adenine, adenosine, guanosine (1-10 mM) were used. There was no inhibitory effect of acetone, glycerol, tetraethylammonium, propilamine, butylamine, aminoacids, spermine, spermidine, ATP, AMP or cAMP. It has been suggested that the inhibitory substances may interact by producing hydrogen bonds from NH-groups with the neutrally or negatively charged groups at the external surface of the muscle membrane in the region with a slow hydrophobicity. As a result, no structural changes required for activation of the sugar transport system occur in the membrane.
在对离体青蛙缝匠肌的实验中，发现胺类和酰胺类物质可抑制由胰岛素、2,4 -二硝基苯酚或钾挛缩诱导的D -木糖转运刺激过程。尿素、乙酰胺、胍、氯化铵、一甲胺、二甲胺、三甲胺或乙胺以及一些二胺（所有这些物质的应用浓度范围均为100 mM）可产生抑制作用。当使用胱胺（20 mM）、色胺、5 -甲氧基色胺（2 mM）以及腺嘌呤、腺苷、鸟苷（1 - 10 mM）时，也获得了类似的效果。丙酮、甘油、四乙铵、丙胺、丁胺、氨基酸、精胺、亚精胺、ATP、AMP或cAMP没有抑制作用。有人提出，抑制性物质可能通过从NH基团与肌肉膜外表面具有缓慢疏水性区域的中性或带负电荷基团形成氢键来相互作用。结果，膜中不会发生激活糖转运系统所需的结构变化。
