Hardy M H, Van Exan R J, Sonstegard K S, Sweeny P R
J Invest Dermatol. 1983 Jan;80(1):27-34. doi: 10.1111/1523-1747.ep12530968.
Skin pieces from 14-day fetal mice were cultivated for 1-10 days prior to fixation and sectioning. Subsequently, sections were studied by light and transmission electron microscopy. In a standard medium the lateral hair follicle walls showed progressive maturation of the basal lamina, while the hair matrix, at the time of a known tissue interaction, showed the formation of gaps in the basal lamina, with heterotypic cell contacts through the gaps. In a vitamin-A enriched medium similar changes occurred, not only at the hair matrix, but also at lateral follicle walls, at the sites of, and prior to, budding and glandular morphogenesis. This study shows that the induction of hair matrix by dermal papilla may perhaps be added to the list of normal tissue interactions in which heterotypic cell contacts occur. It also suggests that vitamin-A induced glandular morphogenesis might come about through a mechanism resembling a normal tissue interaction.
取自14天龄胎鼠的皮肤组织块在固定和切片前培养1至10天。随后，通过光学显微镜和透射电子显微镜对切片进行研究。在标准培养基中，毛囊侧壁的基膜显示出逐渐成熟，而在已知组织相互作用时，毛基质的基膜出现间隙形成，并通过这些间隙形成异型细胞接触。在富含维生素A的培养基中，不仅在毛基质，而且在毛囊侧壁、出芽和腺形态发生部位及其之前，都发生了类似的变化。本研究表明，真皮乳头对毛基质的诱导作用可能可被列入发生异型细胞接触的正常组织相互作用列表中。这也表明，维生素A诱导的腺形态发生可能是通过一种类似于正常组织相互作用的机制实现的。
