胞二磷胆碱和胞二磷乙醇胺对全脑缺血诱导的大鼠脑脂质代谢变化的影响。

Effects of CDPcholine and CDPethanolamine on the alterations in rat brain lipid metabolism induced by global ischemia.

作者信息

Dorman R V, Dabrowiecki Z, Horrocks L A

出版信息

J Neurochem. 1983 Jan;40(1):276-9. doi: 10.1111/j.1471-4159.1983.tb12682.x.

DOI:10.1111/j.1471-4159.1983.tb12682.x

Abstract

The fast turnover pool of rat brain lipids was labeled by intracerebral injection of [3H]acetate. Cerebral ischemia for a duration of 5 min after decapitation caused a 2.2-fold increase in radioactivity in the free fatty acids and loss of more than 20% of the radioactivity from choline and ethanolamine glycerophospholipids. An intracerebral injection of 0.6 mumol each of cytidine diphosphocholine (CDPcholine) and cytidine diphosphoethanolamine (CDPethanolamine) prevented the loss of radioactivity from the glycerophospholipids and decreased the amount of radioactivity in the free fatty acids by 59% as compared with control values and 82% as compared with ischemia values. By GLC assays of the mass of the free fatty acids there was a threefold increase of free fatty acids in ischemic brains. Pretreatment of ischemic brains with CDPcholine and CDPethanolamine reduced the levels of unesterified fatty acids to 60% of the control values. Thus, a prior injection of cytidine nucleotides prevented the release of free fatty acids observed in ischemic brains.

摘要

通过脑内注射[3H]醋酸盐标记大鼠脑脂质的快速周转池。断头后5分钟的脑缺血导致游离脂肪酸中的放射性增加2.2倍，胆碱和乙醇胺甘油磷脂中的放射性损失超过20%。脑内注射0.6 μmol的胞苷二磷酸胆碱（CDP胆碱）和胞苷二磷酸乙醇胺（CDP乙醇胺）可防止甘油磷脂中的放射性损失，与对照值相比，游离脂肪酸中的放射性量减少59%，与缺血值相比减少82%。通过气相色谱法测定游离脂肪酸的质量，缺血脑中游离脂肪酸增加了三倍。用CDP胆碱和CDP乙醇胺预处理缺血脑可将未酯化脂肪酸水平降低至对照值的60%。因此，预先注射胞苷核苷酸可防止在缺血脑中观察到的游离脂肪酸释放。