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[在琼脂中培养的肿瘤细胞(SGS - 3A)的电子显微镜特征]

[Electron microscopic features of neoplastic cells (SGS-3A) cultivated in agar].

作者信息

Meloni M A, Pippia P, Morf-Valluchi M, Cogoli A, Ivaldi G

出版信息

Boll Soc Ital Biol Sper. 1983 Jan 31;59(1):93-9.

PMID:6849699
Abstract

The agar suspension culture method was applied to enrich for neoplastic cells in cultures of a cellular strain (SGS-3) derived from Sarcoma Galliera. This method suppresses the growth of normal cells without impairing colony formation by neoplastic cells. The analysis by electron microscopy of colonies of SGS-3A cells cultured in agar medium shows that cytoplasmatic bridges are present in the regions of cell-cell contact.

摘要

采用琼脂悬浮培养法富集源自加列拉肉瘤的细胞系(SGS - 3)培养物中的肿瘤细胞。该方法可抑制正常细胞生长,同时不影响肿瘤细胞形成集落。对在琼脂培养基中培养的SGS - 3A细胞集落进行电子显微镜分析显示,在细胞 - 细胞接触区域存在细胞质桥。

相似文献

1
[Electron microscopic features of neoplastic cells (SGS-3A) cultivated in agar].[在琼脂中培养的肿瘤细胞(SGS - 3A)的电子显微镜特征]
Boll Soc Ital Biol Sper. 1983 Jan 31;59(1):93-9.
2
[Electron microscopic aspects of a rat tumor induced by sarcoma Galliera cells (SGS-2) cultured in vitro].[体外培养的加列拉肉瘤细胞(SGS-2)诱导的大鼠肿瘤的电子显微镜观察]
Pathologica. 1987 May-Jun;79(1061):299-312.
3
[Karyologic analysis and intercellular adhesion in Galliera sarcoma cells cultivated in vitro: effects of soft agar cloning].[体外培养的加列拉肉瘤细胞的核型分析与细胞间黏附:软琼脂克隆的影响]
Pathologica. 1988 Mar-Apr;80(1066):187-200.
4
[Preliminary reports on homologous and heterologous intercellular adhesion in normal and neoplastic cells cultured in vitro].
Boll Soc Ital Biol Sper. 1981 Jul 30;57(14):1562-7.
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Colony growth in agar by human melanoma cells.人黑色素瘤细胞在琼脂中的集落生长。
Cancer Res. 1981 Mar;41(3):1199-204.
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Indirect immunofluorescence study on the cytoskeleton of normal (FG) and neoplastic (SGS/3A) cadmium treated fibroblasts.正常(FG)和成瘤(SGS/3A)镉处理成纤维细胞细胞骨架的间接免疫荧光研究
Ital J Anat Embryol. 2005 Oct-Dec;110(4):225-36.
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An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture.使用细胞悬浮培养法对培养的角膜缘上皮细胞进行评估。
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Cancer Res. 1993 Apr 15;53(8):1777-82.

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