Sauberman N, Fortier N L, Joshi W, Piotrowski J, Snyder L M
Br J Haematol. 1983 May;54(1):15-28. doi: 10.1111/j.1365-2141.1983.tb02063.x.
Protein crosslinkages are apparent at 215 000-250 000 daltons in electrophoregrams of membranes from hydrogen peroxide treated erythrocytes (British Journal of Haematology, 48, 435, 1981). Hydrogen peroxide is also capable of inducing crosslinkages of identical molecular weights in stage I and II (red) ghosts and in a mixture of purified spectrin and haemoglobin, but not in white ghosts or in either spectrin or haemoglobin alone. Autoradiographic studies using 14C-methaemoglobin and 32P-spectrin confirm the involvement of spectrin and haemoglobin in this reaction. The alpha chains of both proteins are more reactive than the corresponding beta chains: 3 times more reactive in the case of spectrin and 10 times more reactive in haemoglobin. The reaction is almost totally inhibited by NaCN and partially inhibited by N-ethylmaleimide. Direct addition of malondialdehyde to a spectrin-haemoglobin mixture does not result in protein crosslinkage. Metabolic depletion (40 h), in vivo ageing and sucrose dehydration of fresh, normal cells enhance the reaction considerably, whereas in vitro rehydration of xerocytes normalizes their H2O2 sensitivity.
在过氧化氢处理过的红细胞膜的电泳图谱中，蛋白质交联在215000 - 250000道尔顿处明显可见（《英国血液学杂志》，48卷，435页，1981年）。过氧化氢还能够在I期和II期（红色）血影以及纯化的血影蛋白和血红蛋白的混合物中诱导相同分子量的交联，但在白色血影或单独的血影蛋白或血红蛋白中则不能。使用14C - 高铁血红蛋白和32P - 血影蛋白的放射自显影研究证实了血影蛋白和血红蛋白参与了该反应。两种蛋白质的α链比相应的β链更具反应性：血影蛋白的反应性高3倍，血红蛋白的反应性高10倍。该反应几乎完全被NaCN抑制，部分被N - 乙基马来酰亚胺抑制。将丙二醛直接添加到血影蛋白 - 血红蛋白混合物中不会导致蛋白质交联。新鲜正常细胞的代谢耗竭（40小时）、体内老化和蔗糖脱水会显著增强该反应，而干细胞的体外复水会使其对过氧化氢的敏感性恢复正常。
