Groot P H, Scheek L M, Jansen H
Biochim Biophys Acta. 1983 May 16;751(3):393-400. doi: 10.1016/0005-2760(83)90298-9.
Human sera were incubated with rat liver lipase after inactivation of lecithin:cholesterol acyltransferase, and the changes in serum lipoprotein composition were measured. In the presence of liver lipase serum triacylglycerol and phosphatidylcholine were hydrolyzed. The main changes in the concentrations of these lipids were found in the high-density lipoprotein fraction. Subfractionation of high-density lipoprotein by rate-zonal ultracentrifugation showed a prominent decrease in all constituents of high-density lipoprotein2, a smaller decrease in the 'light' high-density lipoprotein3 and an increase in the 'heavy' high-density lipoprotein3. These data support a concept in which liver lipase is involved in high-density lipoprotein2 phospholipid and triacylglycerol catabolism and suggest that as a result of this action high-density lipoprotein2 is converted into high-density lipoprotein3.
在卵磷脂胆固醇酰基转移酶失活后，将人血清与大鼠肝脂肪酶一起孵育，并测定血清脂蛋白组成的变化。在肝脂肪酶存在的情况下，血清三酰甘油和磷脂酰胆碱被水解。这些脂质浓度的主要变化见于高密度脂蛋白部分。通过速率区带超速离心对高密度脂蛋白进行亚分级分离显示，高密度脂蛋白2的所有成分均显著降低，“轻”高密度脂蛋白3的降低幅度较小，而“重”高密度脂蛋白3则增加。这些数据支持了肝脂肪酶参与高密度脂蛋白2磷脂和三酰甘油分解代谢的概念，并表明由于这种作用，高密度脂蛋白2转化为高密度脂蛋白3。
