Finkelstein J N, Maniscalco W M, Shapiro D L
Biochim Biophys Acta. 1983 Jun 2;762(3):398-404. doi: 10.1016/0167-4889(83)90004-6.
The biochemical characteristics of type II alveolar epithelial cells dissociated from adult rabbit lung by instillation of low concentrations of an elastase trypsin mixture are reported. Cells studied immediately (within 4 h) after isolation were found to incorporate the radioactively labelled precursors [U-14C]glucose, [methyl-3H]choline and [3H]palmitate into cellular phosphatidylcholine at rates 2-10-fold higher than previously reported for cells not subject to short-term cell culture. Secretion of phosphatidylcholine was stimulated by beta-adrenergic agonists. Measurement of specific activities of enzymes of phospholipid biosynthesis in subcellular fractions of isolated lung cells showed a significant enrichment of acyl coenzyme A-lysophosphatidylcholine acyltransferase, an enzyme believed to be involved in pulmonary surfactant phosphatidylcholine remodeling, in the endoplasmic reticulum of type II cells. These observations support the utility of freshly isolated type II cells as a model system for the study of the functions of the alveolar epithelium.
报道了通过滴注低浓度弹性蛋白酶 - 胰蛋白酶混合物从成年兔肺中分离出的II型肺泡上皮细胞的生化特性。发现分离后立即（4小时内）研究的细胞将放射性标记的前体[U - 14C]葡萄糖、[甲基 - 3H]胆碱和[3H]棕榈酸酯掺入细胞磷脂酰胆碱的速率比先前报道的未经过短期细胞培养的细胞高2 - 10倍。β - 肾上腺素能激动剂刺激磷脂酰胆碱的分泌。对分离的肺细胞亚细胞组分中磷脂生物合成酶的比活性测量表明，II型细胞内质网中酰基辅酶A - 溶血磷脂酰胆碱酰基转移酶显著富集，该酶被认为参与肺表面活性物质磷脂酰胆碱重塑。这些观察结果支持将新鲜分离的II型细胞用作研究肺泡上皮功能的模型系统的实用性。
