Bingel S A, Sande R D, Newbrey J
Calcif Tissue Int. 1983;35(2):216-24. doi: 10.1007/BF02405034.
This study was performed in order to reexamine the ultrastructural morphology of the chondrocytes in the growth plates of dwarf Alaskan Malamutes and to obtain semiquantitative cytochemical data about the proteoglycans. Growth plates from age-matched dwarf and homozygous nonaffected Alaskan Malamutes were processed for routine transmission electron microscopy and also stained with ruthenium red. Chondrocytes in dwarf plates were observed to occur in clumps or cell nests. Within some of these nests, chondrocytes in the upper half of the zone of chondrocyte proliferation had bizarre shapes ranging from V-shaped to whorled or rounded. These chondrocytes contained profiles of markedly dilated rough endoplasmic reticulum (RER). Material within the RER cisternae stained positively with ruthenium red and was partially digestible with testicular hyaluronidase. The material could, therefore, represent either chondroitin sulfate or hyaluronate. The RER in these dwarf chondrocytes was not oriented parallel to the long axis of the cells; instead, it consisted of irregularly dilated cisternae. Granule counts performed on the zone of chondrocyte proliferation revealed a significant decrease in the number of ruthenium red granules in the interterritorial matrix of dwarf chondrocytes when compared to those of the homozygous nonaffected chondrocytes.
本研究旨在重新审视阿拉斯加矮脚雪橇犬生长板中软骨细胞的超微结构形态，并获取有关蛋白聚糖的半定量细胞化学数据。对年龄匹配的矮脚雪橇犬和纯合未受影响的阿拉斯加雪橇犬的生长板进行常规透射电子显微镜检查，并进行钌红染色。观察到矮脚雪橇犬生长板中的软骨细胞成簇或形成细胞巢。在其中一些细胞巢中，软骨细胞增殖区上半部分的软骨细胞形状怪异，从V形到螺旋形或圆形不等。这些软骨细胞含有明显扩张的粗面内质网（RER）轮廓。RER池内的物质经钌红染色呈阳性，可被睾丸透明质酸酶部分消化。因此，该物质可能代表硫酸软骨素或透明质酸盐。这些矮脚雪橇犬软骨细胞中的RER并非与细胞长轴平行排列；相反，它由不规则扩张的池组成。对软骨细胞增殖区进行的颗粒计数显示，与纯合未受影响的软骨细胞相比，矮脚雪橇犬软骨细胞细胞间基质中钌红颗粒的数量显著减少。
