Brouwer M, Smets L A, Jongsma A P
Cancer Res. 1983 Jun;43(6):2884-8.
Two subclones of L1210 murine leukemia (L1210-46.1 and L1210-56.3) were isolated in the absence of selective agents. Subclone 56.3 appeared to be more sensitive than was subclone 46.1 to treatment with dexamethasone, 1-beta-D-arabinofuranosyl cytosine, vincristine, and X-irradiation. No differences between the parent cells and the two subclones could be observed in population-doubling time, cloning efficiency, number of chromosomes, and tumorigenic potential in DBA/2 mice. The subclones did not differ in the per cell number of glucocorticoid receptor sites. Animal experiments revealed an increase in life span of 65% in mice inoculated with cells from subclone 46.1 and of 130% of mice with subclone 56.3 after treatment with 1-beta-D-arabinofuranosylcytosine. The present results indicate that the L1210 wild-type murine leukemia cells contained stable subpopulations with a different but collateral sensitivity to various cytotoxic treatments. It is postulated that differences in drug sensitivity between cells are partly determined by cellular properties which are independent of the mechanism of action of any specific treatment.
在没有选择剂的情况下分离出L1210小鼠白血病的两个亚克隆（L1210 - 46.1和L1210 - 56.3）。亚克隆56.3似乎比46.1亚克隆对用地塞米松、1-β-D-阿拉伯呋喃糖基胞嘧啶、长春新碱和X射线照射更敏感。在群体倍增时间、克隆效率、染色体数目以及在DBA/2小鼠中的致瘤潜力方面，未观察到亲代细胞与这两个亚克隆之间存在差异。这两个亚克隆在每个细胞的糖皮质激素受体位点数量上没有差异。动物实验显示，在用1-β-D-阿拉伯呋喃糖基胞嘧啶处理后，接种46.1亚克隆细胞的小鼠寿命延长了65%，接种56.3亚克隆细胞的小鼠寿命延长了130%。目前的结果表明，L1210野生型小鼠白血病细胞包含稳定的亚群，这些亚群对各种细胞毒性治疗具有不同但相关的敏感性。据推测，细胞之间药物敏感性的差异部分由细胞特性决定，这些特性独立于任何特定治疗的作用机制。
