White C A, Vass S W, Kennedy J F
Carbohydr Res. 1983 Apr 1;114(2):201-7. doi: 10.1016/0008-6215(83)88187-7.
The Elson-Morgan assay for 2-amino-2-deoxyhexoses, despite its many modifications, can still give variable results because of slight variations in reaction conditions. An automated method is reported which uses microgram samples and provides greater sensitivity than hitherto possible. The use of sodium orthophosphate and optimisation of the concentrations of the reagents provide conditions that are more stable, and results that are more reliable, than any previously reported.
用于检测2-氨基-2-脱氧己糖的埃尔森-摩根检测法,尽管有许多改进,但由于反应条件的细微变化,仍可能给出不同的结果。本文报道了一种自动化方法,该方法使用微克级样本,比以往任何方法都具有更高的灵敏度。使用正磷酸钠并优化试剂浓度所提供的条件比以往任何报道的条件都更稳定,结果也更可靠。