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对杜邦自动化学分析仪中使用的钠和钾直接电位测定法的评估。

Evaluation of a direct potentiometric method for sodium and potassium used in the Du Pont aca.

作者信息

Koch D D, Parrish D, Ladenson J H

出版信息

Clin Chem. 1983 Jun;29(6):1090-2.

PMID:6851101
Abstract

We evaluated an accessory that enables the Du Pont aca discrete analyzer to measure Na+ and K+ by direct potentiometry (aca/ISE). Na+ and K+ gave linear responses in both the blood and urine modes, with no carryover. No interfering species were identified in the blood mode. Intra-assay and interassay precision for Na+ and K+ were more than adequate and analytical recoveries comparable to those with flame photometry were found for plasma. The Na+ and K+ values for plasma were comparable with those obtained by use of another direct potentiometric analyzer (the Orion SS-30), but slightly higher than flame-photometric values. Samples from patients with hyperlipemia and multiple myeloma gave clinically relevant values with the aca/ISE, but the flame photometric values were artefactually low. Whole blood could not be accurately analyzed. The aca/ISE is a precise, easy-to-use instrument, which gives values for plasma similar to those obtained with direct potentiometric analyzers. For analysis of urine, certain precautions are necessary.

摘要

我们评估了一种附件,它能使杜邦aca离散分析仪通过直接电位分析法(aca/离子选择性电极法)测量钠离子(Na+)和钾离子(K+)。在血液和尿液模式下,Na+和K+均呈现线性响应,且无残留。在血液模式下未发现干扰物质。Na+和K+的批内和批间精密度均足够,且血浆的分析回收率与火焰光度法相当。血浆中的Na+和K+值与使用另一种直接电位分析仪(奥立龙SS-30)获得的值相当,但略高于火焰光度法的值。高脂血症和多发性骨髓瘤患者的样本使用aca/离子选择性电极法可得出临床相关值,但火焰光度法的值人为地偏低。全血无法准确分析。aca/离子选择性电极法是一种精确、易于使用的仪器,其血浆值与直接电位分析仪获得的值相似。对于尿液分析,需要采取某些预防措施。

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