Shlaes D M, Lederman M, Chmielewski R, Tweardy D, Wolinsky E
Chest. 1983 Jun;83(6):885-9. doi: 10.1378/chest.83.6.885.
We have observed five patients for whom the presence of fibers of elastin in potassium hydroxide (KOH) preparations of sputum represented the first evidence of necrotizing pulmonary disease. In four cases, the discovery of elastin fibers in sputum provided additional evidence supporting initiation or modification of antibiotic therapy. Necrotizing disease was confirmed in all cases by autopsy or by the development of cavitation on chest x-ray film. Cytochemical staining, electron microscopy, and elastase digestion all suggest that the refractile fibers seen on KOH wet mount of sputum are elastin. The test, first described in 1846, is simple to perform, requires little experience to read, and may be a valuable adjunct to the chest roentgenogram in the diagnosis of pulmonary parenchymal destruction.
我们观察了5例患者，在其痰液的氢氧化钾（KOH）制剂中发现弹性蛋白纤维是坏死性肺病的首个证据。在4例病例中，痰液中弹性蛋白纤维的发现为启动或调整抗生素治疗提供了额外证据。所有病例均通过尸检或胸部X光片上出现空洞证实为坏死性疾病。细胞化学染色、电子显微镜检查和弹性蛋白酶消化均表明，在痰液KOH湿片中看到的折光性纤维是弹性蛋白。该检测于1846年首次描述，操作简单，判读几乎不需要经验，在诊断肺实质破坏方面可能是胸部X光检查的有价值辅助手段。
