卤虫胚胎微注射的聚腺苷酸结合蛋白在非洲爪蟾卵母细胞中的亚细胞分布。

Subcellular distribution in Xenopus laevis oocytes of a microinjected poly(A)-binding protein from Artemia salina gastrulae.

作者信息

De Herdt E, Marbaix G, Tencer R, Slegers H

出版信息

Eur J Biochem. 1983 May 16;132(3):623-7. doi: 10.1111/j.1432-1033.1983.tb07409.x.

DOI:10.1111/j.1432-1033.1983.tb07409.x

PMID:6852017

Abstract

The poly(A)-binding protein P38 of non-polysomal mRNP from Artemia salina gastrulae was labelled by reductive methylation and microinjected into the cytoplasm of Xenopus laevis oocytes. The labelled protein has a half-life of approximately 20 h and accumulated in the nucleus of the oocyte. The kinetics of accumulation reached a plateau at about 15 h after microinjection. P38 accumulates in the nucleus to a final concentration 3.15 times higher than that reached by free diffusion. This fact suggests that P38, a cytoplasmic poly(A)-binding protein, might also play some role in the nucleus of the cell.

摘要

卤虫胚胎原肠胚中非多聚核糖体信使核糖核蛋白颗粒（mRNP）的多聚腺苷酸结合蛋白P38通过还原甲基化进行标记，并显微注射到非洲爪蟾卵母细胞的细胞质中。标记的蛋白质半衰期约为20小时，并在卵母细胞核中积累。积累动力学在显微注射后约15小时达到平台期。P38在细胞核中积累的最终浓度比自由扩散所达到的浓度高3.15倍。这一事实表明，作为一种细胞质多聚腺苷酸结合蛋白，P38可能在细胞核中也发挥着某种作用。