Cheng J B, Townley R G
Eur J Pharmacol. 1983 Mar 25;88(2-3):269-72. doi: 10.1016/0014-2999(83)90017-1.
Using [3H]QNB, we demonstrated that GTP and Gpp(NH)p increased muscarinic receptor high affinity sites in bovine tracheal muscle preparations; however, neither the dissociation constant of [3H]QNB binding sites nor pulmonary beta-adrenergic receptor sites was altered. The GTP effect on increasing the receptor sites was relatively small (16%), sensitive (ED50 0.48 microM) and specific (Gpp(NH)p greater than GTP = GDP greater than GMP much greater than ATP). Mg2+ potentiated this increase by up to 94.8%, whereas Na+, K+ and Ca2+ had no such effect.
使用[3H]QNB，我们证明了GTP和Gpp(NH)p增加了牛气管肌肉制剂中的毒蕈碱受体高亲和力位点；然而，[3H]QNB结合位点的解离常数和肺β-肾上腺素能受体位点均未改变。GTP对增加受体位点的作用相对较小（16%）、敏感（ED50为0.48 microM）且具有特异性（Gpp(NH)p＞GTP = GDP＞GMP＞＞ATP）。Mg2+可使这种增加增强高达94.8%，而Na+、K+和Ca2+则无此作用。
