Faulkner J A, Markley J M, McCully K K, Watters C R, White T P
Exp Neurol. 1983 Jun;80(3):682-96. doi: 10.1016/0014-4886(83)90318-7.
Grafting of 3-g extensor digitorum longus (EDL) muscles of cats may be made with (i) severence of the nerve with spontaneous reinnervation, termed standard grafts (ii) severence of the nerve with reinnervation facilitated by anastomosis of the nerve, termed nerve-anastomosed grafts; and (iii) preservation of the nerve, termed nerve-intact grafts. In previous studies, standard grafts developed a maximum isometric tetanic tension (P0) that was 22% of the value for control EDL muscles. We hypothesized that the low values of P0 resulted from incomplete reinnervation of muscle fibers. To test this hypothesis, EDL muscles were grafted in cats with nerves intact and with nerves anastomosed. In standard grafts differences were observed in both structure and function at 120 compared with 240 days after grafting. Characteristics of the nerve-intact and nerve-anastomosed grafts did not change significantly between 120 and 240 days and the data were pooled for comparisons with control EDL muscles. Nerve-anastomosed and nerve-intact grafts developed P0 values that were 34 and 64% of the control values, respectively. Nerve-intact grafts had a mass and fiber cross-sectional area not different from control EDL muscles. Compared with control values, all grafts had fewer fibers, more connective tissue, lower absolute and normalized P0, reduced capillary density, and increased fatigability. The greater P0 of nerve-intact compared with standard and nerve-anastomosed grafts supported our hypothesis that the degree of reinnervation is a factor that limits graft development. The presence of a necrotic core and the low tension development of even the nerve-intact grafts suggested that revascularization is a significant limitation as well.
可采用以下三种方式对猫的3克趾长伸肌（EDL）进行移植：（i）切断神经使其自发再支配，称为标准移植；（ii）切断神经并通过神经吻合促进再支配，称为神经吻合移植；（iii）保留神经，称为神经完整移植。在先前的研究中，标准移植所产生的最大等长强直张力（P0）为对照EDL肌肉值的22%。我们推测P0值较低是由于肌纤维再支配不完全所致。为验证这一假设，将EDL肌肉移植到神经完整和神经吻合的猫体内。与移植后240天相比，在120天时观察到标准移植在结构和功能上均存在差异。神经完整移植和神经吻合移植的特征在120天至240天之间没有显著变化，因此将数据合并用于与对照EDL肌肉进行比较。神经吻合移植和神经完整移植所产生的P0值分别为对照值的34%和64%。神经完整移植的质量和纤维横截面积与对照EDL肌肉无差异。与对照值相比，所有移植的纤维数量均较少，结缔组织较多，绝对和标准化的P0较低，毛细血管密度降低，疲劳性增加。与标准移植和神经吻合移植相比，神经完整移植的P0值更高，这支持了我们的假设，即再支配程度是限制移植发育的一个因素。坏死核心的存在以及即使是神经完整移植的低张力发展表明，血管再形成也是一个重要的限制因素。
