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广黑点瘤姬蜂:营养水平对一种小蜂类昆虫寄生虫体外幼虫生长的影响

Brachymeria lasus: effects of nutrient level on in vitro larval growth of a chalcid insect parasite.

作者信息

Thompson S N

出版信息

Exp Parasitol. 1983 Jun;55(3):312-9. doi: 10.1016/0014-4894(83)90028-0.

DOI:10.1016/0014-4894(83)90028-0
PMID:6852170
Abstract

Exponential weight gain equations and growth rates were determined during larval development of Brachymeria lasus reared aseptically on chemically defined media with varying amino acid and glucose levels. The rate of weight gain increased from 0 to 2% dietary glucose and mean growth rate increased from 160 to 260 micrograms/mg x day. Similar results were observed for larvae reared on media containing 1 to 4% free amino acids. At higher nutrient levels, growth rate and rate of weight gain declined and maximal growth occurred at dietary osmolalities of 500-700 mOsm/kg. A further experiment utilizing nutritionally inert L-glucose suggested that this osmolality range does not reflect the optimal for growth but rather the point at which the detrimental effects of dietary osmotic pressure exceed the nutritive benefit of the dietary ingredients.

摘要

在以不同氨基酸和葡萄糖水平的化学成分确定培养基无菌饲养的短角瘤姬蜂幼虫发育过程中,测定了指数体重增加方程和生长速率。体重增加速率在饮食葡萄糖从0增加到2%时上升,平均生长速率从160微克/毫克×天增加到260微克/毫克×天。在含有1%至4%游离氨基酸的培养基上饲养的幼虫也观察到类似结果。在较高营养水平下,生长速率和体重增加速率下降,最大生长出现在饮食渗透压为500 - 700毫渗摩尔/千克时。利用无营养活性的L - 葡萄糖进行的进一步实验表明,这个渗透压范围并不反映生长的最佳状态,而是饮食渗透压的有害影响超过饮食成分营养益处的点。

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