Albini A, Richter H, Pontz B F
FEBS Lett. 1983 Jun 13;156(2):222-6. doi: 10.1016/0014-5793(83)80500-6.
Fragments derived from human plasma fibronectin by enzymatic degradation were tested in the Boyden chamber for chemotactic activity towards various fibroblast strains. The results provide clear evidence that the chemotactic activity is restricted to a defined region of the fibronectin molecule which is the same for various fibroblast strains. The active domain is localized between the collagen binding site and the major heparin binding site, about 170 kDa apart from the N-terminal and about 70 kDa from the C-terminal ends of the two subunit peptide chains.
通过酶促降解从人血浆纤连蛋白衍生而来的片段，在博伊登小室中针对各种成纤维细胞系进行趋化活性测试。结果提供了明确的证据，表明趋化活性局限于纤连蛋白分子的一个特定区域，该区域对各种成纤维细胞系而言是相同的。活性结构域位于胶原结合位点和主要肝素结合位点之间，与两条亚基肽链的N端相距约170 kDa，与C端相距约70 kDa。
