Slavík J
FEBS Lett. 1983 Jun 13;156(2):227-30. doi: 10.1016/0014-5793(83)80501-8.
The distribution of pH inside the yeast Endomyces magnusii was measured at 1 micron resolution using different external pH values. In a neutral buffer the pH of the cytoplasm was 6.7-7.2 in the center, decreasing to 6.0 toward the periphery of the cell. A decrease of external pH was followed by a gradual uniform decrease of internal pH. Using a comparison of the 'pH map' with phase-contrast picture of the same cell, the pH of the vacuoles was estimated to be 5.5-5.6.
使用不同的外部pH值，以1微米的分辨率测量了大酵母内的pH分布。在中性缓冲液中，细胞质中心的pH值为6.7-7.2，向细胞周边逐渐降至6.0。外部pH值降低后，内部pH值逐渐均匀下降。通过将“pH图”与同一细胞的相差图片进行比较，估计液泡的pH值为5.5-5.6。
