糖皮质激素及其他药物杀伤的S49淋巴瘤细胞中的DNA片段化

DNA fragmentation in S49 lymphoma cells killed with glucocorticoids and other agents.

作者信息

Vedeckis W V, Bradshaw H D

出版信息

Mol Cell Endocrinol. 1983 May;30(2):215-27. doi: 10.1016/0303-7207(83)90049-7.

Abstract

The effects of glucocorticoids on DNA integrity in the mouse S49 lymphoma cell line were assessed. DNA cleavage at the internucleosomal regions was observed, and this response was correlated to the dose of hormone used and the time of treatment. Also, an apparent steroid specificity was observed: internucleosomal cleavage was associated only with treatment of the cells with glucocorticoids. Cells treated with dibutyryl cyclic AMP (which also causes lymphocytolysis) also exhibited DNA cleavage. However, when cells were killed with various DNA synthesis inhibitors and other lethal agents, the same DNA cleavage pattern was observed. Furthermore, new protein synthesis did not seem to be required, since cells killed with puromycin and cycloheximide also exhibited internucleosomal DNA fragmentation. Although DNA fragmentation may not be a specific early effect of glucocorticoid-mediated lymphocytolysis, it may be the final, irrevocable step in this complex process.

摘要

评估了糖皮质激素对小鼠S49淋巴瘤细胞系中DNA完整性的影响。观察到核小体间区域的DNA裂解，并且这种反应与所用激素的剂量和处理时间相关。此外，还观察到明显的类固醇特异性：核小体间裂解仅与用糖皮质激素处理细胞有关。用二丁酰环磷腺苷（也可引起淋巴细胞溶解）处理的细胞也表现出DNA裂解。然而，当用各种DNA合成抑制剂和其他致死剂杀死细胞时，观察到相同的DNA裂解模式。此外，似乎不需要新的蛋白质合成，因为用嘌呤霉素和环己酰亚胺杀死的细胞也表现出核小体间DNA片段化。虽然DNA片段化可能不是糖皮质激素介导的淋巴细胞溶解的特异性早期效应，但它可能是这个复杂过程中的最终、不可逆转的步骤。