Brennan M J, Millis A J, Mann D, Fritz K E
Dev Biol. 1983 Jun;97(2):391-7. doi: 10.1016/0012-1606(83)90095-7.
Nontransformed cultures of vascular smooth muscle cells proliferate until they form a confluent sheet of cells. Subsequently, the cells become reorganized to form multicellular nodules that are loosely attached to the substrate. The formation of nodules is facilitated by the addition of medium conditioned by nodular cultures. Nodulation is inhibited by the addition of fibronectin. Fibronectins derived from monolayer culture conditioned medium or from plasma are maximally effective while fibronectin isolated from nodular cell conditioned medium is inactive. Analysis by NaDodSO4-polyacrylamide gel electrophoresis reveals that the nodular cell fibronectin has a molecular weight that is about 20-30 kd less than that of monolayer cell fibronectin. Further, nodular cell conditioned medium contains an activity that can convert both plasma fibronectin and monolayer cell fibronectin to the lower molecular weight correlated with the loss of biological activity.
未转化的血管平滑肌细胞培养物会不断增殖，直至形成一层汇合的细胞。随后，细胞会重新组织形成多细胞结节，这些结节松散地附着在基质上。添加经结节培养物处理过的培养基有助于结节的形成。添加纤连蛋白会抑制结节形成。源自单层培养条件培养基或血浆的纤连蛋白效果最佳，而从结节细胞条件培养基中分离出的纤连蛋白则无活性。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析发现，结节细胞纤连蛋白的分子量比单层细胞纤连蛋白小约20 - 30kd。此外，结节细胞条件培养基含有一种活性物质，它能将血浆纤连蛋白和单层细胞纤连蛋白都转化为与生物活性丧失相关的较低分子量形式。
