Kirby M L, Stewart D E
Dev Biol. 1983 Jun;97(2):433-43. doi: 10.1016/0012-1606(83)90100-8.
Using interspecific grafting of neural crest between quail and chick embryos, it was determined that the cardiac ganglia originate from the cranial region (somites 1-2) of the vagal neural crest (somites 1-7). Neuronal uptake of [3H]choline was used as an index of neuronal development in the chick atrium. Normal uptake was found to be quite high between Days 8 and 14 of incubation. Following extirpation of neural crest over somites 1 to 3 at stages 8 to 10, neuronal uptake in 8-day chick atrium was decreased by 25-60% depending on the stage at which the lesion was performed. It is thought that the residual uptake represents preganglionic terminals from the dorsal motor nucleus of the vagus. Embryos with extirpations of neural crest over somites 1-3 performed at stage 9 showed the greatest decrease of neuronal choline uptake and did not live beyond 11 days of incubation. However, hearts from embryos with partial lesions (performed at stage 10) were treated on incubation Days 12 and 15 for demonstration of acetylcholinesterase in the subepicardial plexus. These hearts showed much less extensive neural plexus with sparse, small cardiac ganglia.
通过鹌鹑和鸡胚胎间神经嵴的种间移植，确定心脏神经节起源于迷走神经嵴（第1 - 7体节）的颅部区域（第1 - 2体节）。[3H]胆碱的神经元摄取被用作鸡心房神经元发育的指标。发现在孵化第8天至14天期间正常摄取量相当高。在第8至10阶段切除第1至3体节上方的神经嵴后，8日龄鸡心房中的神经元摄取量根据损伤时的阶段降低了25% - 60%不等。据认为，残余摄取量代表来自迷走神经背运动核的节前终末。在第9阶段进行第1 - 3体节上方神经嵴切除的胚胎，其神经元胆碱摄取量下降最大，且在孵化11天后无法存活。然而，对部分损伤（在第10阶段进行）的胚胎心脏在孵化第12天和15天进行处理，以显示心外膜下丛中的乙酰胆碱酯酶。这些心脏显示出神经丛范围小得多，心脏神经节稀疏且小。
