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须癣毛癣菌中的脲酶活性。

Urease activity in Trichophyton mentagrophytes.

作者信息

Sanyal A K, Banerjee A B

出版信息

Folia Microbiol (Praha). 1983;28(2):82-6. doi: 10.1007/BF02877361.

DOI:10.1007/BF02877361
PMID:6852689
Abstract

Urease activity was detected in the dermatophyte Trichophyton mentagrophytes cells at early exponential phase of growth. Specific activity of urease decreased with culture age. At exogenous urea concentrations above 2 mM formation of urease was inhibited. The pH optimum lay at 7-7.5, the Km being 14 mM. No urease activity could be detected in cell-free culture fluid of T. mentagrophytes. No endo- or exocellular urease activity could be detected in a T. rubrum strain grown with or without urea.

摘要

在皮肤癣菌须癣毛癣菌生长的指数生长期早期,可检测到脲酶活性。脲酶的比活性随培养时间而降低。当外源尿素浓度高于2 mM时,脲酶的形成受到抑制。最适pH值为7 - 7.5,米氏常数为14 mM。在须癣毛癣菌的无细胞培养液中未检测到脲酶活性。在用或不用尿素培养的红色毛癣菌菌株中,均未检测到细胞内或细胞外脲酶活性。

相似文献

1
Urease activity in Trichophyton mentagrophytes.须癣毛癣菌中的脲酶活性。
Folia Microbiol (Praha). 1983;28(2):82-6. doi: 10.1007/BF02877361.
2
The differentiation of Trichophyton rubrum and T. mentagrophytes by use of Christensen's urea broth.利用克里斯滕森尿素肉汤对红色毛癣菌和须癣毛癣菌进行鉴别
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[Comparative study of physiological properties for identification of Trichophyton rubrum and Trichophyton mentagrophytes. Pigment production, urease and lipase activity, growth in pepton solution and by hair perforation test (author's transl)].用于鉴定红色毛癣菌和须癣毛癣菌的生理特性比较研究。色素产生、脲酶和脂肪酶活性、在蛋白胨溶液中的生长情况以及毛发穿孔试验(作者译)
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引用本文的文献

1
Factors affecting growth and urease production by Trichophyton spp.影响毛癣菌属生长及脲酶产生的因素
Mycopathologia. 1996;135(2):109-13. doi: 10.1007/BF00436460.

本文引用的文献

1
Pigment production in the differentiation of Trichophyton mentagrophytes and Trichophyton rubrum.须发癣菌和红色毛癣菌分化过程中的色素产生
Mycologia. 1949 May-Jun;41(3):291-302.
2
Nutritional studies of the dermatophytes with special reference to Trichophyton Megnini Blanchard 1896 and Trichophyton gallinae (Megnin 1881) comb. Nov.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
Observations on submerged growth and deamination of amino acids by dermatophytes.关于皮肤癣菌在水下生长及氨基酸脱氨作用的观察
J Invest Dermatol. 1958 Apr;30(4):197-9.
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An improved method for detection of urea hydrolysis by fungi.
J Infect Dis. 1957 Jul-Aug;101(1):51-61. doi: 10.1093/infdis/101.1.51.
6
In vitro hair cultures for differentiating between atypical isolates of Trichophyton mentagrophytes and Trichophyton rubrum.用于区分须癣毛癣菌和红色毛癣菌非典型分离株的体外毛发培养
Mycopathol Mycol Appl. 1957 Mar 25;8(1):3-17. doi: 10.1007/BF02053114.
7
The differentiation of Trichophyton mentagrophytes from T. rubrum by a simple urease test.通过简单的尿素酶试验区分须癣毛癣菌和红色毛癣菌。
Sabouraudia. 1967 Feb;5(3):189-93.
8
Enzymatic studies with pathogenic fungi.致病真菌的酶学研究。
Dermatol Int. 1965 Apr-Jun;4(2):72-8. doi: 10.1111/j.1365-4362.1965.tb05127.x.
9
The relative importance of urease test and hair-perforation test in routine differentiation to Trichophyton rubrum and T. mentagrophyte.尿素酶试验和毛发穿孔试验在常规鉴别红色毛癣菌和须癣毛癣菌中的相对重要性。
Bull Calcutta Sch Trop Med. 1968 Apr;16(2):43-5.
10
The use of nutritional tests for the differentiation of dermatophytes.
Sabouraudia. 1977 Jul;15(2):141-50. doi: 10.1080/00362177785190221.