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福氏耐格里阿米巴对大鼠神经母细胞瘤细胞培养物的细胞致病性:扫描电子显微镜研究

Cytopathogenicity of Naegleria fowleri for rat neuroblastoma cell cultures: scanning electron microscopy study.

作者信息

Marciano-Cabral F, John D T

出版信息

Infect Immun. 1983 Jun;40(3):1214-7. doi: 10.1128/iai.40.3.1214-1217.1983.

DOI:10.1128/iai.40.3.1214-1217.1983
PMID:6852919
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348179/
Abstract

Neuroblastoma cells were inoculated with Naegleria fowleri Lee and examined for cytopathology at various periods post-inoculation by scanning electron microscopy. By 18 h post-inoculation, approximately 50% of neuroblastoma cells were nonviable, as evidenced by trypan blue exclusion and light microscopic examination. This cytopathology resulted from piecemeal consumption of target cells mediated by a sucker apparatus extending from the surface of N. fowleri.

摘要

将福氏耐格里阿米巴(Lee株)接种到神经母细胞瘤细胞上,并在接种后的不同时间段通过扫描电子显微镜检查细胞病理学变化。接种后18小时,通过台盼蓝排斥试验和光学显微镜检查证明,约50%的神经母细胞瘤细胞失去活性。这种细胞病理学变化是由福氏耐格里阿米巴表面伸出的吸盘装置介导的对靶细胞的逐步消耗所致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af92/348179/1a1edcad4e8c/iai00141-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af92/348179/da5fd1f81936/iai00141-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af92/348179/1a1edcad4e8c/iai00141-0376-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af92/348179/da5fd1f81936/iai00141-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af92/348179/1a1edcad4e8c/iai00141-0376-a.jpg

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本文引用的文献

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2
The fixation, dehydration, drying and coating of cultured cells of SEM.扫描电子显微镜(SEM)培养细胞的固定、脱水、干燥及镀膜
J Microsc. 1981 Aug;123(Pt 2):121-31. doi: 10.1111/j.1365-2818.1981.tb01288.x.
3
Primary amoebic meningoencephalitis caused by Naegleria. Two new cases, response to amphotericin B, and a review.由耐格里属阿米巴原虫引起的原发性阿米巴脑膜脑炎。两例新病例、对两性霉素B的反应及文献综述
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Parasitol Res. 2018 Mar;117(3):937-941. doi: 10.1007/s00436-018-5779-9. Epub 2018 Jan 29.
4
Naegleria fowleri after 50 years: is it a neglected pathogen?福氏耐格里阿米巴50年之后:它是一种被忽视的病原体吗?
J Med Microbiol. 2016 Sep;65(9):885-896. doi: 10.1099/jmm.0.000303. Epub 2016 Jul 4.
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BMC Genomics. 2014 Jun 19;15(1):496. doi: 10.1186/1471-2164-15-496.
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