Runyan T E, McCombs W B, Holton O D, McCoy C E, Morgan M P
Invest Ophthalmol Vis Sci. 1983 Jun;24(6):687-96.
Human ciliary body epithelial cells have been maintained in vitro and have been partially characterized by the determination of growth rate, morphology, and ultrastructural parameters. The dissection technique employed allows the separation of pure ciliary body epithelium with a predominance of cells being from the nonpigmented layer. Growth curves indicate this cell population follows a prolonged rate of growth compared to other primary cell cultures. Loss of pigment granules noted by light microscopy were documented by morphometric analysis of electron micrographs. Thirty-two percent of the cultures attempted were successful. Maintenance of these cells in vitro may provide a means for studying their enzyme systems, growth factors, reactions to various stimuli, and the effects of this cell population on other intraocular tissues.
人睫状体上皮细胞已在体外培养，并通过测定生长速率、形态和超微结构参数进行了部分特性鉴定。所采用的解剖技术能够分离出以非色素层细胞为主的纯睫状体上皮。生长曲线表明，与其他原代细胞培养物相比，这群细胞的生长速率较为缓慢。光学显微镜下观察到的色素颗粒丢失情况，通过电子显微镜照片的形态计量分析得以记录。所尝试的培养中有32%取得成功。在体外培养这些细胞可能为研究其酶系统、生长因子、对各种刺激的反应以及这群细胞对其他眼内组织的影响提供一种手段。
