Houston L L
J Biol Chem. 1983 Jun 10;258(11):7208-12.
4-Azidophenyl-beta-D-galactopyranoside and 4-diazophenyl-beta-D-galactopyranoside were used to inactivate ricin. Galactose, but not glucose, protected against inactivation as measured by the retention of the ability of ricin to bind to Bio-Gel A, a galactose-containing gel. Nearly complete inhibition of binding to Bio-Gel A, to monosaccharides, or to cell surface receptors could be achieved by reaction of ricin with either label, but neither label impaired the ability of the A chain to inhibit translation in vitro. The diazonium salt-modified ricin still inhibited cellular protein synthesis, but ricin modified by the photoactivated label was 280 times less efficient than ricin in inhibition of cellular protein synthesis.
4-叠氮苯基-β-D-吡喃半乳糖苷和4-重氮苯基-β-D-吡喃半乳糖苷被用于使蓖麻毒素失活。如通过蓖麻毒素与含半乳糖的凝胶Bio-Gel A结合能力的保留来衡量，半乳糖而非葡萄糖可保护蓖麻毒素不被失活。蓖麻毒素与任一标记物反应可几乎完全抑制其与Bio-Gel A、单糖或细胞表面受体的结合，但两种标记物均未损害A链在体外抑制翻译的能力。重氮盐修饰的蓖麻毒素仍能抑制细胞蛋白质合成，但经光活化标记物修饰的蓖麻毒素在抑制细胞蛋白质合成方面的效率比未修饰的蓖麻毒素低280倍。
