Matsuzawa S, Itoh Y, Kimura H, Kobayashi R, Miyauchi C
J Immunol Methods. 1983 May 27;60(1-2):189-96. doi: 10.1016/0022-1759(83)90346-0.
A microtiter latex antiglobulin test (LAGT) with heavy-and-stable latex particles was developed. Antibodies of weak avidity, such as rabbit antisera to DNP or digoxin were much enhanced by the LAGT, whereas the method only slightly promoted strong avidity antibody, such as that found in rabbit antisera to human chorionic gonadotrophin. A microtiter latex agglutination-inhibition test (LAGIT) proved to be an effective tool for detection of weak antigenic substances such as digoxin and DNP.
开发了一种使用重且稳定的乳胶颗粒的微量滴定乳胶抗球蛋白试验(LAGT)。LAGT可显著增强弱亲和力抗体,如兔抗DNP或地高辛抗血清,而该方法对强亲和力抗体的促进作用较小,如兔抗人绒毛膜促性腺激素抗血清中的抗体。微量滴定乳胶凝集抑制试验(LAGIT)被证明是检测地高辛和DNP等弱抗原物质的有效工具。