Bentley A M, Wallis M
J Endocrinol. 1983 May;97(2):221-8. doi: 10.1677/joe.0.0970221.
Anterior pituitary glands from female rats were dispersed enzymically in the absence of dopamine. Dispersed cells (10(6)-10(7)) were layered onto columns containing Bio-Gel P-2 and were then perifused for 3 h with Dulbecco's Modified Eagle's Medium. The prolactin content of the perifusate and cell homogenates was determined by radioimmunoassay. Prolactin secretion during the third hour of perifusion increased as the loading of cells increased. However, the increase was not linear, and when secretion rate per 10(6) cells was calculated it was found that increased loading decreased the rate, which fell to a plateau of 1.3 +/- 0.1 (S.E.M.) ng/min per 10(6) cells at a loading of about 8 x 10(6) cells from 3.8 +/- 0.1 ng/min per 10(6) cells for a loading of 10(6) cells. This cell-density dependence of the rate of prolactin secretion in the perifusion system may be due to intercellular contact since the isolation of the tissue removes the influence of hypothalamic factors, while localized build up of prolactin (potentially causing direct autoregulation on the lactotroph) seems unlikely because of the continuous flow of medium.
在无多巴胺的情况下，对雌性大鼠的垂体前叶进行酶解分散处理。将分散的细胞（10⁶ - 10⁷个）铺在装有生物凝胶P - 2的柱上，然后用杜氏改良伊格尔培养基进行3小时的灌流。通过放射免疫分析法测定灌流液和细胞匀浆中的催乳素含量。在灌流的第三个小时，催乳素分泌量随着细胞接种量的增加而增加。然而，这种增加并非呈线性，当计算每10⁶个细胞的分泌率时发现，接种量增加会使分泌率降低，对于接种量为10⁶个细胞时，分泌率为3.8±0.1 ng/分钟每10⁶个细胞，而当接种量约为8×10⁶个细胞时，分泌率降至1.3±0.1（标准误）ng/分钟每10⁶个细胞的平台期。在灌流系统中，催乳素分泌率对细胞密度的这种依赖性可能是由于细胞间接触，因为组织的分离消除了下丘脑因子的影响，而由于培养基的持续流动，催乳素的局部积累（可能对催乳细胞产生直接的自动调节作用）似乎不太可能。
