Bentley A M, Wallis M
J Endocrinol. 1982 Sep;94(3):317-26. doi: 10.1677/joe.0.0940317.
The effects of omperidone on prolactin secretion from the anterior pituitary glands of female rats were studied. A perifusion system and radioimmunoassay were used to study secretion; static incubations, and incorporation of [3H]leucine were used to measure biosynthesis. During perifusion, intact anterior lobes showed a constant rate of prolactin secretion for up to 5h (after a 90-min preincubation to stabilize the tissue). Incorporation studies revealed an increase in protein synthesis in perifused hemipituitary glands. When glands were treated with 10nM-domperidone , prolactin secretion began to decline after the first hour, reaching a maximum of 40-50% inhibition after a further 90 min. Growth hormone secretion showed no such decline. Inhibition of prolactin secretion continued for up to 2h after withdrawal of the drug. Treatment with 100nM-dopamine resuled in a more rapid inhibition of secretion, but the effect was reversed on withdrawal of the catecholamine. The prolactin content of perifused hemipituitary glands was measured after treatment with domperidone; the contents of control and treated glands did not differ, but were depleted compared with hemipituitary glands which had not been perifused.
研究了奥哌啶对雌性大鼠垂体前叶催乳素分泌的影响。采用灌流系统和放射免疫分析法研究分泌情况；采用静态孵育和[3H]亮氨酸掺入法测定生物合成。在灌流过程中，完整的垂体前叶在长达5小时内显示出恒定的催乳素分泌速率（经过90分钟的预孵育以稳定组织）。掺入研究表明，灌流的半垂体中蛋白质合成增加。当腺体用10nM多潘立酮处理时，催乳素分泌在第一小时后开始下降，再过90分钟后抑制率最高可达40-50%。生长激素分泌未出现此类下降。停药后催乳素分泌抑制持续长达2小时。用100nM多巴胺处理导致分泌抑制更快，但在撤除儿茶酚胺后作用逆转。用多潘立酮处理后测量灌流半垂体中的催乳素含量；对照腺体和处理腺体的含量没有差异，但与未灌流的半垂体相比有所减少。
