Davis P H, Berlandi F J, Dullude G R, Griffin R M, Matson W R, Zink E W
Am Ind Hyg Assoc J. 1978 Jun;39(6):480-90. doi: 10.1080/0002889778507793.
A method for the measurement of parts per billion levels of total arsenic in urine and blood is described. Samples are wet ashed with a mixture of HNO3, HCIO4, and H2SO4 acids. Ashed materials are subjected to a reductillationTM procedure to reduce As (V) to As (III) and to separate arsenic from the sample matrix. Collected arsenic is then quantitated by anodic stripping voltammetry (ASV) at a gold film electrode. ASV analysis time is only 2 minutes. By simultaneous reductillation of 4 samples, ppb arsenic determinations can be accomplished at a rate of about 12 per hour. The method is as accurate, precise and reliable at the nanogram level as the more universally accepted colorimetric techniques are at the microgram and milligram levels. For replicate analysis of real samples, method precision ranged from +/- 1.4 ppb at the 5 ppb level to +/- 0.96 ppb at the 25 ppb level. Accuracy is estimated at +/- 6% over the range 5 to 500 ppb arsenic.
本文描述了一种测量尿液和血液中十亿分之一水平总砷的方法。样品用硝酸、高氯酸和硫酸的混合物进行湿式灰化。灰化后的物质经过还原程序，将五价砷还原为三价砷，并从样品基质中分离出砷。然后在金膜电极上通过阳极溶出伏安法（ASV）对收集到的砷进行定量。ASV分析时间仅为2分钟。通过同时对4个样品进行还原，每小时大约可以完成12次十亿分之一砷的测定。该方法在纳克水平上的准确性、精密度和可靠性与更广泛接受的比色技术在微克和毫克水平上相当。对于实际样品的重复分析，方法精密度在5 ppb水平时为±1.4 ppb，在25 ppb水平时为±0.96 ppb。在5至500 ppb砷的范围内，估计准确度为±6%。
