Mølster A O, Gjerdet N R, Alho A, Bang G
Acta Orthop Scand. 1983 Jun;54(3):366-73. doi: 10.3109/17453678308996586.
Rigid intramedullary nailing of the right tibia after osteotomy with resection of the fibula was performed on 48 male Wistar rats weighting 300-400 g. Nailing was performed with 1.4-mm nails after reaming. Nail stiffness was similar to that of intact tibias. The left leg remained unoperated as control. The animals were sacrificed after 4, 8, 12 or 16 weeks, and both tibiae were tested in a 3-point bending test. Of the 35 animals with the nail in situ, nine animals showed non-union at the time of testing. At 8 weeks stiffness had reached normal values, while strength and deflection showed about half of normal values. Poor strength and small deflectability combined with near normal stiffness may be taken as evidence that Wolf's law is valid also in newly formed tissues of fracture healing; the protection given by a rigid nail makes normal strength and pliability unnecessary.
对48只体重300 - 400克的雄性Wistar大鼠进行右侧胫骨截骨并切除腓骨后行刚性髓内钉固定术。扩髓后用1.4毫米的钉子进行钉固定。钉子的刚度与完整胫骨相似。左腿未手术作为对照。在4、8、12或16周后处死动物，对双侧胫骨进行三点弯曲试验。在35只钉子在位的动物中，9只动物在测试时出现骨不连。8周时刚度达到正常值，而强度和挠度约为正常值的一半。强度差、可挠度小且刚度接近正常，这可作为沃尔夫定律在骨折愈合新形成组织中也有效的证据；刚性钉子提供的保护使得正常强度和柔韧性不再必要。
