Immunochemical studies of human kininogen.

Antisera directed against the isolated heavy and light chains of high MW kininogen were used for immunochemical studies of high MW and low MW kininogens. The heavy chain region of high MW kininogen share identical immunologic determinants indicating similar structures. The light chain region of high MW kininogen is immunologically distinct from low MW kininogen and is therefore unique to high MW kininogen. Consequently the anti-heavy chain antibody could be used in radioimmunoassays to quantitate total kininogens antigens, whereas the anti-light chain antibody could be used as specific tool to quantitate high MW kininogen.