McCoy K L, Engel D, Clagett J
Am J Pathol. 1983 Jul;112(1):18-26.
The rapidly fatal autoimmune disease accompanied by pneumonitis in the mutant mouse known as motheaten is caused by an autosomal recessive gene. The proliferative capacity and production rate of splenic mononuclear phagocytes at different maturational stages, defined by morphologic criteria, were examined by two in vitro tritiated thymidine administration protocols and radioautography. The replicative rate of splenic promonocytes from 3-week-old normal mice was found to approximate that of adult bone marrow cells. Monocytes, macrophages, and previously described macrophagelike cells from motheaten mice had an accelerated rate of maturation in vitro, compared with similar cells from normal mice. These differences in the production rates could be attributed to the shorter period of time that promonocytes from the mutant mice are in the S phase of the cell cycle. Evidence, also, verified that the macrophagelike cells were derived from promonocytes via the monocyte-macrophage compartment. The unusual in vitro growth characteristics of the splenic mononuclear phagocytes from motheaten mice could be a manifestation of an in vivo abnormality of this lineage that could contribute to the development of pulmonary disease in these animals.
在名为“食母生”的突变小鼠中，伴有肺炎的快速致死性自身免疫疾病是由常染色体隐性基因引起的。通过两种体外氚化胸腺嘧啶核苷给药方案和放射自显影法，研究了根据形态学标准定义的不同成熟阶段脾单核吞噬细胞的增殖能力和产生速率。发现3周龄正常小鼠脾前单核细胞的复制速率与成年骨髓细胞相近。与正常小鼠的类似细胞相比，食母生小鼠的单核细胞、巨噬细胞以及先前描述的类巨噬细胞在体外成熟速率加快。这些产生速率的差异可归因于突变小鼠的前单核细胞处于细胞周期S期的时间较短。证据还证实，类巨噬细胞是通过单核细胞-巨噬细胞区室从前单核细胞衍生而来。食母生小鼠脾单核吞噬细胞异常的体外生长特性可能是该细胞系体内异常的一种表现，这可能导致这些动物肺部疾病的发生。
