Reisser D, Boutillon M M, Clément J
Ann Nutr Metab. 1983;27(3):252-60. doi: 10.1159/000176667.
Labelled thoracic-duct lymph was collected from nonfasting rats with a bile fistula after simultaneous intraduodenal infusions of bile labelled with [1-2 3H] cholesterol and a nutritive mixture containing [4-14C] cholesterol. The gastrointestinal tract, feces, chylomicrons and infranatants were analysed. Both biliary and exogenous cholesterol were absorbed by lymphatic way but the recovery of 3H labelling in total lymph was markedly higher than that of 14C activity. This fact might be due to different rates of cholesterol exchanges from the two origins with the nonlabelled cholesterol present in the enterocytes and further exchanges of the enterocytes cholesterol with plasma cholesterol. Most of radioactivity was detected in chylomicrons. The relative [3H] and [14C] cholesterol specific activities were always low; thus when a little exogenous cholesterol is brought the major part of lymph cholesterol had an endogenous--other than biliary--source.
给有胆瘘的非禁食大鼠同时十二指肠内输注用[1-2³H]胆固醇标记的胆汁和含[4-¹⁴C]胆固醇的营养混合物后，收集标记的胸导管淋巴。对胃肠道、粪便、乳糜微粒和上清液进行分析。胆汁胆固醇和外源性胆固醇均通过淋巴途径吸收，但总淋巴中³H标记的回收率明显高于¹⁴C活性的回收率。这一事实可能是由于来自两个来源的胆固醇与肠细胞中存在的未标记胆固醇的交换速率不同，以及肠细胞胆固醇与血浆胆固醇的进一步交换所致。大部分放射性在乳糜微粒中检测到。相对的[³H]和[¹⁴C]胆固醇比活性始终较低；因此，当引入少量外源性胆固醇时，淋巴胆固醇的主要部分具有内源性——而非胆汁源性——来源。
