分离的大鼠肝细胞中电生性钠-丙氨酸共转运的偶联比率
Coupling ratio of electrogenic Na+-alanine cotransport in isolated rat hepatocytes.
作者信息
Kristensen L O, Folke M
出版信息
Biochem J. 1983 Feb 15;210(2):621-4. doi: 10.1042/bj2100621.
Abstract
Na+-alanine cotransport across the cell membrane in isolated rat hepatocytes was studied. Changes in the cell membrane potential associated with the transport of alanine interfere with determination of the Na+-alanine coupling ratio of the cotransport. With valinomycin present to 'clamp' the cell membrane potential, a coupling ratio of 1:1 for the Na+-alanine influx was obtained.
摘要
研究了分离的大鼠肝细胞中Na⁺-丙氨酸跨细胞膜的转运。与丙氨酸转运相关的细胞膜电位变化会干扰Na⁺-丙氨酸共转运耦合比的测定。在存在缬氨霉素以“钳制”细胞膜电位的情况下，Na⁺-丙氨酸内流的耦合比为1:1。
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