Kubilus J, Baden H P
Biochim Biophys Acta. 1983 Jun 29;745(3):285-91. doi: 10.1016/0167-4838(83)90060-2.
The deimination of guanidyl groups of peptides, proteins and other arginine-containing compounds is catalyzed by enzymes found in mammalian brain and epidermis. In cow, the brain and epidermal enzymes differ kinetically and physically, but both may be quantitated by measuring the production of benzoyl citrulline ethyl ester from benzoyl-arginine ethyl ester. The brain enzyme has been purified to apparent homogeneity, as judged by the presence of only one 85,000 dalton band in purified preparations when examined by SDS-polyacrylamide gel electrophoresis. An antibody raised to this band precipitates pure and partially purified brain enzyme but not partially purified epidermal enzyme, using the Ouchterlony technique. The antibody bound to an insoluble matrix removes brain enzyme activity from solution but not epidermal enzyme activity. The Km of the brain enzyme for benzoyl-arginine ethyl ester is about 0.33 mM.
肽、蛋白质及其他含精氨酸化合物的胍基脱氨作用由哺乳动物大脑和表皮中的酶催化。在牛体内，大脑和表皮中的酶在动力学和物理性质上有所不同，但二者都可通过测量苯甲酰精氨酸乙酯生成苯甲酰瓜氨酸乙酯的量来进行定量分析。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测，纯化后的大脑酶在纯化制剂中仅出现一条85,000道尔顿的条带，据此判断其已纯化至表观均一。采用免疫双扩散技术，针对该条带产生的抗体可沉淀纯的及部分纯化的大脑酶，但不能沉淀部分纯化的表皮酶。与不溶性基质结合的抗体可从溶液中去除大脑酶活性，但不能去除表皮酶活性。大脑酶对苯甲酰精氨酸乙酯的米氏常数约为0.33 mM。
