Airoldi L, Bonfanti M, Benfenati E, Tavecchia P, Fanelli R
Biomed Mass Spectrom. 1983 May;10(5):334-7. doi: 10.1002/bms.1200100505.
N-Nitrosodiethanolamine metabolism was studied in order to clarify the mechanism(s) by which this compound elicits its carcinogenic effect. Samples of 24 h urine from rats given a single dose of N-nitrosodiethanolamine were collected. Gas chromatographic thermal energy analyses showed the presence of only one compound containing the nitroso moiety besides N-nitrosodiethanolamine. This compound was identified as the acidic derivative N-(2-hydroxyethyl)-N-carboxymethylnitrosamine by comparison with an authentic standard by means of gas chromatographic mass spectrometric analysis of the trimethylsilyl or pentafluorobenzyl esters. The amount of N-(2-hydroxyethyl)-N-carboxymethylnitrosamine excreted in the 24 h urine was about 6% of the N-nitrosodiethanolamine administered.
为阐明N-亚硝基二乙醇胺产生致癌作用的机制，对其代谢情况进行了研究。收集了单次给予N-亚硝基二乙醇胺的大鼠24小时尿液样本。气相色谱热能分析表明，除N-亚硝基二乙醇胺外，仅存在一种含亚硝基部分的化合物。通过对三甲基硅烷基酯或五氟苄基酯进行气相色谱-质谱分析，并与标准品比较，将该化合物鉴定为酸性衍生物N-(2-羟乙基)-N-羧甲基亚硝胺。24小时尿液中排出的N-(2-羟乙基)-N-羧甲基亚硝胺的量约为所给予的N-亚硝基二乙醇胺的6%。
